Author: Jacob Peter Matson; Amy M. House; Gavin D. Grant; Huaitong Wu; Joanna Perez; Jeanette Gowen Cook
Title: Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence Document date: 2019_2_22
ID: dsbucda9_53
Snippet: Cells were plated for live cell imaging with G0 by contact inhibition and restimulation in Flourobrite DMEM (Gibco) with 10% FBS and 2 mM L-glutamine in #1.5 glass bottom plates (Cellvis) in a humidified enclosure at 37° C with 5% CO2. Movie started 6.5 hours after plating, cells were imaged for 72 hours with images every 10 minutes. Cells were imaged on a Nikon Ti Eclipse inverted microscope with a 20x (NA 0.75) Apochromat dry objective lens wi.....
Document: Cells were plated for live cell imaging with G0 by contact inhibition and restimulation in Flourobrite DMEM (Gibco) with 10% FBS and 2 mM L-glutamine in #1.5 glass bottom plates (Cellvis) in a humidified enclosure at 37° C with 5% CO2. Movie started 6.5 hours after plating, cells were imaged for 72 hours with images every 10 minutes. Cells were imaged on a Nikon Ti Eclipse inverted microscope with a 20x (NA 0.75) Apochromat dry objective lens with the Nikon Perfect Focus System. The camera was an Ando Zyla 4.2 sCMOS detector with 12 bit resolution, filters were from Chroma: (excitation; beam splitter; emission filter) CFP -436/20 nm; 455 nm; 480/40 nm, YFP -500/20 nm; 515 nm; 535/30 nm; and mCherry -560/40 nm; 585 nm; 630/75 nm. Images were collected with Nikon NIS-Elements AR software.
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