Selected article for: "coronavirus protein and infection cause"

Author: Natalia B. Hubbs; Mareena M. Whisby-Pitts; Jonathan L. McMurry
Title: Kinetic Analysis of Bacteriophage Sf6 Binding to Outer Membrane Protein A Using Whole Virions
  • Document date: 2019_1_2
  • ID: ktds6nla_33
    Snippet: The data presented herein suggest that the previously reported differences in Sf6 infection efficiencies seen in vivo and the differences in Sf6 genome ejection efficiencies in vitro [23] are not based solely on the kinetics of receptor binding. There are no significant kinetic differences between Sf6 binding to the various OmpA-TMs, nor are there any significant changes when temperature is varied, even though both factors have been shown to caus.....
    Document: The data presented herein suggest that the previously reported differences in Sf6 infection efficiencies seen in vivo and the differences in Sf6 genome ejection efficiencies in vitro [23] are not based solely on the kinetics of receptor binding. There are no significant kinetic differences between Sf6 binding to the various OmpA-TMs, nor are there any significant changes when temperature is varied, even though both factors have been shown to cause changes in infection efficiency. Our results are similar to those in another study in which the authors use SPR and demonstrate that amino acid substitutions in the coronavirus receptor binding protein do not greatly affect the overall kinetic parameters when binding to the human receptor CD26 [35] .

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