Author: Stefania Marzinotto; Catia Mio; Adriana Cifu'; Roberto Verardo; Corrado Pipan; Claudio Schneider; Francesco Curcio
Title: A streamlined approach to rapidly detect SARS-CoV-2 infection, avoiding RNA extraction Document date: 2020_4_11
ID: mi6w8ppe_6
Snippet: (which was not peer-reviewed) The copyright holder for this preprint DTT, 20U/µL Reverse Transcriptase, 5 µL sample and nuclease-free water were mixed and brought to a total volume of 20 µL. Samples were mixed with Droplet Generator Oil for Probes (Bio-Rad, catalog number 1863005) and droplets were generated on the automated droplet generator QX200™ Droplet Generator (Bio-Rad). PCR amplification was performed by the Veriti® Thermal Cycler (.....
Document: (which was not peer-reviewed) The copyright holder for this preprint DTT, 20U/µL Reverse Transcriptase, 5 µL sample and nuclease-free water were mixed and brought to a total volume of 20 µL. Samples were mixed with Droplet Generator Oil for Probes (Bio-Rad, catalog number 1863005) and droplets were generated on the automated droplet generator QX200™ Droplet Generator (Bio-Rad). PCR amplification was performed by the Veriti® Thermal Cycler (ThermoFisher Scientific) with annealing at 55°C and standard thermal cycling conditions. Droplets were read on the QX200™ Droplet Reader (Bio-Rad) and reactions with less than 10'000 droplets were repeated. Data were analyzed using the QuantaSoft™ 1.7.4 Software (Bio-Rad).
Search related documents:
Co phrase search for related documents- droplet reaction and PCR amplification: 1
- nuclease free water and PCR amplification: 1, 2, 3, 4, 5
- nuclease free water and total volume: 1, 2
- PCR amplification and total volume: 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12
Co phrase search for related documents, hyperlinks ordered by date