Author: Jonathan L Schmid-Burgk; David Li; David Feldman; Mikolaj Slabicki; Jacob Borrajo; Jonathan Strecker; Brian Cleary; Aviv Regev; Feng Zhang
Title: LAMP-Seq: Population-Scale COVID-19 Diagnostics Using a Compressed Barcode Space Document date: 2020_4_8
ID: 68ps3uit_16
Snippet: has not yet been experimentally validated, and that additional false negatives may arise during the RT-LAMP reaction stage. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.025635 doi: bioRxiv preprint viral sequence. To further increase specificity, viral sequences can be filtered for sequence portions that are non-overlapping with primer sequences. In addition, we expect that this .....
Document: has not yet been experimentally validated, and that additional false negatives may arise during the RT-LAMP reaction stage. The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.06.025635 doi: bioRxiv preprint viral sequence. To further increase specificity, viral sequences can be filtered for sequence portions that are non-overlapping with primer sequences. In addition, we expect that this novel multiplexing-LAMP strategy is unlikely to suffer from barcode cross-contamination originating from template switching events at the PCR stage, as two template switching events would be required in order to create a sequencing-compatible amplicon.
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