Author: Bingyi Yang; Justin Lessler; Huachen Zhu; Chaoqiang Jiang; Jonathan M. Read; James A Hay; Kin On Kwok; Ruiyin Shen; Yi Guan; Steven Riley; Derek A.T. Cummings
Title: Life course exposures continually shape antibody profile and risk of seroconversion to influenza Document date: 2020_1_16
ID: cyxn7dpw_52
Snippet: We collected serum from participants at two time points, i.e. baseline (2009 to 2011) and follow-up (2014-2015) . Serum from both visits were available from 777 participants out of 2,767 total participants (across both visits, table S1), of which 763 participants had interpretable titers for all 21 H3N2 strains ( Fig For each strain-individual pair, HAI titers were determined by two-fold serial dilutions from 1:10 to 1:1280 conducted in 96-well m.....
Document: We collected serum from participants at two time points, i.e. baseline (2009 to 2011) and follow-up (2014-2015) . Serum from both visits were available from 777 participants out of 2,767 total participants (across both visits, table S1), of which 763 participants had interpretable titers for all 21 H3N2 strains ( Fig For each strain-individual pair, HAI titers were determined by two-fold serial dilutions from 1:10 to 1:1280 conducted in 96-well microtiter plates with 0.5% turkey erythrocytes. Sera from the two visits were tested side by side on the same plate, and confirmatory samples collected in 2014 sera were tested on a separate plate. The reciprocal of the highest dilution where hemagglutination does not occur is reported as the titer. There were 28 missing (for 14 individuals and 5 strains) out of 32,636 strain-individual-visit combinations, which were due to inadequate sera remaining or inconclusive readings.
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