Selected article for: "fss bead multi frame tag and os cell"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_82
    Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/478040 doi: bioRxiv preprint Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the + FSS multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 7 seconds for a total of 217 (of 280) second.....
    Document: The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. . https://doi.org/10.1101/478040 doi: bioRxiv preprint Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the + FSS multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 7 seconds for a total of 217 (of 280) seconds total post puromycin addition. This movie shows the same cell from Movie S3, but now zoomed in to accentuate a frameshifting site splitting (red arrows). Scale bar, 10 µm.

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