Selected article for: "fss bead multi frame tag and scale bar"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_84
    Snippet: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the + FSS 2x multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 10 seconds for a total of 100 seconds pre-FRAP and 790 seconds post-FRAP. Control spot (far left red arrow) and two frameshifting sites (center arrows) were tracked through time to measur.....
    Document: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the + FSS 2x multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 10 seconds for a total of 100 seconds pre-FRAP and 790 seconds post-FRAP. Control spot (far left red arrow) and two frameshifting sites (center arrows) were tracked through time to measure their fluorescence (0 frame translation) after photobleaching. Scale bar, 10 µm.

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