Selected article for: "open human reading frame and reading frame"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_96
    Snippet: AgeI. The open reading frame encoding human XXLb1/AlexX (Abramowitz et al., 2004; Aydin et al., 2009 ) was amplified from U-2 OS cells cDNA with the primers: 5'-GTT GTC ATA TGG GCG TGC GCA ACT -3'; 5'-GAT GTA GCT AGC CTA GAA GCA GCA GGC GGT G -3'. The amplified XXLb1/AlexX was flanked with NsiI and NheI, and then inserted into the C-terminal region of the FSS-MF, FSS-2xMF and FSS-revMF to obtain FSS-MF-AlexX (i.e. the +FSS multi-frame tag), FSS-2.....
    Document: AgeI. The open reading frame encoding human XXLb1/AlexX (Abramowitz et al., 2004; Aydin et al., 2009 ) was amplified from U-2 OS cells cDNA with the primers: 5'-GTT GTC ATA TGG GCG TGC GCA ACT -3'; 5'-GAT GTA GCT AGC CTA GAA GCA GCA GGC GGT G -3'. The amplified XXLb1/AlexX was flanked with NsiI and NheI, and then inserted into the C-terminal region of the FSS-MF, FSS-2xMF and FSS-revMF to obtain FSS-MF-AlexX (i.e. the +FSS multi-frame tag), FSS-2xMF-AlexX (i.e. the +FSS 2x multi-frame tag), and FSS-revMF-AlexX (i.e. the +FSS reverse multi-frame tag), respectively. To All rights reserved. No reuse allowed without permission.

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