Author: Chin-Yi Chu; Xing Qiu; Matthew N. McCall; Lu Wang; Anthony Corbett; Jeanne Holden-Wiltse; Christopher Slaunwhite; Qian Wang; Christopher Anderson; Alex Grier; Steven R. Gill; Gloria S. Pryhuber; Ann R. Falsey; David J. Topham; Mary T. Caserta; Edward E. Walsh; Thomas J Mariani
Title: Insufficiency in airway interferon activation defines clinical severity to infant RSV infection Document date: 2019_5_20
ID: bx49tbui_52
Snippet: For establishing Air-liquid interface cultures, 100,000 cells were seeded on rat tail collagen I (34.5µg/mL) coated Transwell inserts (12 well PET membrane, 0.4μm pore size, 12 mm diameter). After 24-48 hours, culture medium was changed to 1:1 mixture of BEGM/DMEM. When cells reached confluence, with appropriate resistance (≥ 300 Ohms*cm2), they were transferred to ALI by removing the apical medium. Upon transition to ALI, cells were maintain.....
Document: For establishing Air-liquid interface cultures, 100,000 cells were seeded on rat tail collagen I (34.5µg/mL) coated Transwell inserts (12 well PET membrane, 0.4μm pore size, 12 mm diameter). After 24-48 hours, culture medium was changed to 1:1 mixture of BEGM/DMEM. When cells reached confluence, with appropriate resistance (≥ 300 Ohms*cm2), they were transferred to ALI by removing the apical medium. Upon transition to ALI, cells were maintained in PneumaCult-ALI medium containing supplements and hydrocortisone, according to manufacturer's instructions. ALI cultures were differentiated for 10-14 days at ALI before virus infection.
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