Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_117
Snippet: Oligo FSS RNA co-transfection An uncapped RNA oligo containing the FSS from HIV-1 was synthesized from IDTDNA with the following RNA sequence: 5'-CUG GCC UUC CCU UGU GGG AAG GCC AGA UCU UCC CUA AAA AA -3'. Co-transfection of FSS-MF-AlexX and the RNA oligo was carried out via bead-loading, as describe above. Briefly, 1 µg plasmid FSS-MF-AlexX construct DNA, 100 µg/ml of fluorescently labeled Fab, 250 µg/ml of purified (GCN4) scFv-sfGFP or 1 µg.....
Document: Oligo FSS RNA co-transfection An uncapped RNA oligo containing the FSS from HIV-1 was synthesized from IDTDNA with the following RNA sequence: 5'-CUG GCC UUC CCU UGU GGG AAG GCC AGA UCU UCC CUA AAA AA -3'. Co-transfection of FSS-MF-AlexX and the RNA oligo was carried out via bead-loading, as describe above. Briefly, 1 µg plasmid FSS-MF-AlexX construct DNA, 100 µg/ml of fluorescently labeled Fab, 250 µg/ml of purified (GCN4) scFv-sfGFP or 1 µg of (GCN4) scFv-sfGFP plasmid, 1 or 4 µg of RNA oligo, and 33 µg/ml of purified MCP-HaloTag protein were mixed with PBS to a final volume of ~4 µl of PBS.
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