Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_86
Snippet: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the HA multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), HA Fab Cy3 (Ch2 green, all ORFs), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired post harringtonine every 120 seconds for a total of 3480 seconds. Scale bar, 10 µm......
Document: Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the HA multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), HA Fab Cy3 (Ch2 green, all ORFs), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired post harringtonine every 120 seconds for a total of 3480 seconds. Scale bar, 10 µm.
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