Long noncoding RNA AVAN promotes antiviral innate immunity by interacting with TRIM25 and enhancing the transcription of FOXO3a

Chengcai Lai; Lihui Liu; Qinghua Liu; Sijie Cheng; Keyu Wang; Lingna Zhao; Min Xia; Cheng Wang; Hongjing Gu; Yueqiang Duan; Zhongpeng Zhao; Lili Zhang; Ziyang Liu; Jianjun Luo; Jianxun Song; Penghui Yang; Runsheng Chen; Xiliang Wang

The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. Table S11 ). Most of these divergent 194 genes were associated with antiviral innate immune responses and inflammatory diseases according to Reactome pathway analysis ( Fig 4B) and FunDO diseases analysis ( Fig S5) . 196 Notably, genes associated with immune system IFN signaling and cytokine signaling were 197 markedly up-regulated in cells with ectopic AVAN expression compared with control cells, 198 including chemokines and ISGs (IFN stimulated genes) (Fig 4C) . These significantly 199 up-regulated genes, including MX1, ISG15, IFIT2, OASL, IFIM3, TNFAIP3, CXCL2 and 200 CCL5, were confirmed by qRT-PCR (Fig 4D) , and these were abolished after AVAN 201 knockdown upon IAV-BJ501 infection ( Fig 4E) . These data again reveal that AVAN strongly 202 participates in antiviral innate immune processes and neutrophil chemotaxis. The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. Fig S6K) . 236 In addition, we knocked down FOXO3a in AVAN-overexpressing A549 cells and found that 237 knockdown abrogated AVAN-induced effects on IL-8 expression during IAV-BJ501 infection 238 ( Fig 5P) . These data provide evidence that AVAN plays a critical role in regulating FOXO3a 239 All rights reserved. No reuse allowed without permission.