Author: Tong, Suxiang; Lingappa, Jairam R.; Chen, Qi; Shu, Bo; LaMonte, Ashley C.; Cook, Byron T.; Birge, Charryse; Wang Chern, Shur-wern; Liu, Xin; Galloway, Renee; Le Quynh, Mai; Wai, Fu Ng; Yang, Jyh-Yuan; Butany, Jagdish; Comer, James A.; Monroe, Stephan S.; Beard, Suzanne R.; Ksiazek, Thomas G.; Erdman, Dean; Rota, Paul A.; Pallansch, Mark A.; Anderson, Larry J.
Title: Direct Sequencing of SARS-Coronavirus S and N Genes from Clinical Specimens Shows Limited Variation Cord-id: xbu18zdr Document date: 2004_9_15
ID: xbu18zdr
Snippet: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients—1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/ cell-culture-isolate pairs from 6 patients. We identified
Document: Severe acute respiratory syndrome-associated coronavirus (SARS-CoV) emerged, in November 2002, as a novel agent causing severe respiratory illness. To study sequence variation in the SARS-CoV genome, we determined the nucleic acid sequence of the S and N genes directly from clinical specimens from 10 patients—1 specimen with no matched SARS-CoV isolate, from 2 patients; multiple specimens from 3 patients; and matched clinical-specimen/ cell-culture-isolate pairs from 6 patients. We identified 3 nucleotide substitutions that were most likely due to natural variation and 2 substitutions that arose after cell-culture passage of the virus. These data demonstrate the overall stability of the S and N genes of SARS-CoV over 3 months during which a minimum of 4 generations for transmission events occurred. These findings are a part of the expanding investigation of the evolution of how this virus adapts to a new host.
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