Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo Document date: 2018_4_6
ID: 1pbshnw9_69
Snippet: Intriguingly, this region of the FTH1 transcripts corresponds precisely to the iron responsive element (IRE), which is a well-studied structural element that regulates translation in an iron-dependent fashion (58) . We scanned our list of AGO-associated stem-loops for other IRE containing genes, and found Ferritin light chain (FTL) mRNA was also producing AGO-associated stem-loops from its IRE region (Fig. 7E) . Furthermore, we identified AGO-ass.....
Document: Intriguingly, this region of the FTH1 transcripts corresponds precisely to the iron responsive element (IRE), which is a well-studied structural element that regulates translation in an iron-dependent fashion (58) . We scanned our list of AGO-associated stem-loops for other IRE containing genes, and found Ferritin light chain (FTL) mRNA was also producing AGO-associated stem-loops from its IRE region (Fig. 7E) . Furthermore, we identified AGO-associated stem-loops supported by icSHAPE data from the mouse homologs of both of these genes, Fth1 and Ftl (Figs. S8A-E) . Finally, we confirmed the presence of the FTH1 pre-miRNA-like structure by northern blotting (Fig. 7D) . Therefore, processing of IREs from ferritin genes into AGO-associated stem-loops is conserved in mammals.
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