Author: Ian M. Silverman; Sager J. Gosai; Nicholas Vrettos; Shawn W. Foley; Nathan D. Berkowitz; Zissimos Mourelatos; Brian D. Gregory
Title: Isolation and sequencing of AGO-bound RNAs reveals characteristics of mammalian stem-loop processing in vivo Document date: 2018_4_6
ID: 1pbshnw9_78
Snippet: From a further examination of pre-miRNA-seq reads that did not map to miRBase, we identified AGO-associated stem-loops derived from mRNAs (Fig. 5A ). We found that these were enriched in the 5' UTR of these transcripts, which suggests they may play a similar role to miR-3618 in the 5' UTR of human DGCR8 (Figs. 5B-C) . Furthermore, we found that these stem-loops had a broader size distribution than known miRNAs and were oligo-uridylated, suggest.....
Document: From a further examination of pre-miRNA-seq reads that did not map to miRBase, we identified AGO-associated stem-loops derived from mRNAs (Fig. 5A ). We found that these were enriched in the 5' UTR of these transcripts, which suggests they may play a similar role to miR-3618 in the 5' UTR of human DGCR8 (Figs. 5B-C) . Furthermore, we found that these stem-loops had a broader size distribution than known miRNAs and were oligo-uridylated, suggesting they are processed by TUTases (Figs. 5D-G) . RNA structure prediction algorithms and in vivo structure probing data from mouse ES cells provide strong evidence that these regions of mRNAs form stem-loops (Fig. S5) .
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