Selected article for: "chain reaction and genomic dna"
Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress
  • Document date: 2019_6_20
    • ID: dt0b7jnu_46
    Snippet: . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint Genomic DNA was extracted from tail using extraction E buffer, TPS and N buffer. 25 µl of E buffer and 7 ul of TPS buffer were added and stored room temperature at 10 min. Then tails were heated at 95 o C in 5 min and 25 µl of N buff.....
    Document: . CC-BY-NC-ND 4.0 International license is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the author/funder. It . https://doi.org/10.1101/675785 doi: bioRxiv preprint Genomic DNA was extracted from tail using extraction E buffer, TPS and N buffer. 25 µl of E buffer and 7 ul of TPS buffer were added and stored room temperature at 10 min. Then tails were heated at 95 o C in 5 min and 25 µl of N buffer was added. Polymerase chain reaction was used for examined of genotype using the following primers: forward: 5'-TGCGAGGCACGATATGGCGA-3', reverse: 5'-AGCGCACCTGTCACTTTCCTGC-3'. The amplicon with 1200 and 1700 bp were detected in wild-type and Gm15441-null mice, respectively.

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