Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress Document date: 2019_6_20
ID: dt0b7jnu_59
Snippet: Whole cell extracts were prepared from mouse liver tissue or mouse primary hepatocytes using RIPA buffer supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo-Fisher Scientific) and 1 mM PMSF. Protein concentrations were determined using the Pierce BCA Protein Assay Kit (Pierce, Rockford, IL). Forty µg of protein was loaded per lane on a 4-12%.....
Document: Whole cell extracts were prepared from mouse liver tissue or mouse primary hepatocytes using RIPA buffer supplemented with Halt Protease and Phosphatase Inhibitor Cocktail (Thermo-Fisher Scientific) and 1 mM PMSF. Protein concentrations were determined using the Pierce BCA Protein Assay Kit (Pierce, Rockford, IL). Forty µg of protein was loaded per lane on a 4-12%
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