Longitudinal characteristics of lymphocyte responses and cytokine profiles in the peripheral blood of SARS-CoV-2 infected patients

Jing Liu; Sumeng Li; Jia Liu; Boyun Liang; Xiaobei Wang; Hua Wang; Wei Li; Qiaoxia Tong; Jianhua Yi; Lei Zhao; Lijuan Xiong; Chunxia Guo; Jin Tian; Jinzhuo Luo; Jinghong Yao; Ran Pang; Hui Shen; Cheng Peng; Ting Liu; Qian Zhang; Jun Wu; Ling Xu; Sihong Lu; Baoju Wang; Zhihong Weng; Chunrong Han; Huabing Zhu; Ruxia Zhou; Helong Zhou; Xiliu Chen; Pian Ye; Bin Zhu; Shengsong He; Yongwen He; Shenghua Jie; Ping Wei; Jianao Zhang; Yinping Lu; Weixian Wang; Li Zhang; Ling Li; Fengqin Zhou; Jun Wang; Ulf Dittmer; Mengji Lu; Yu Hu; Dongliang Yang; Xin Zheng

Laboratory confirmation of the SARS-CoV-2 was performed by local CDC according to Chinese CDC protocol. Throat-swab specimens were collected from all patients and the samples were maintained in viral-transport medium for laboratory testing. An infection with other respiratory viruses including influenza A virus, influenza B virus, Coxsackie virus, respiratory syncytial virus, parainfluenza virus and enterovirus was excluded by real-time RT-PCR. Specimens, including sputum or alveolar lavatory fluid, blood, urine, and feces, were cultured to identify pathogenic bacteria or fungi that may be associated with the SARS-CoV-2 infection. The specific IgG and IgM of Chlamydia pneumonia and Mycoplasma pneumonia were detected by chemiluminescence immunoassay. The lymphocyte test kit (Beckman Coulter Inc., FL, USA) was used for lymphocyte subset analysis. Plasma cytokines (IL2, IL4, IL6, IL10, TNFα and IFNγ ) were detected with human Th1/2 cytokine kit II (BD Ltd., Franklin lakes, NJ, USA). All tests are performed according to the product manual. All rights reserved. No reuse allowed without permission. author/funder, who has granted medRxiv a license to display the preprint in perpetuity. author/funder, who has granted medRxiv a license to display the preprint in perpetuity.