Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_28
Snippet: To investigate the RNA binding property of prepared pxMNPs, RNA binding assays were performed by incubating 2 μg RNA molecules with 20 μg pcMNPs in 200 μL lysis/binding buffer. As shown in Figure2A, more than 90% of RNA was absorbed by the pcMNPs within 10 min. In contrast, significant amount RNA molecules have been observed using a commercialized RNA extraction kit under similar conditions. More importantly, no aggregation has been observed w.....
Document: To investigate the RNA binding property of prepared pxMNPs, RNA binding assays were performed by incubating 2 μg RNA molecules with 20 μg pcMNPs in 200 μL lysis/binding buffer. As shown in Figure2A, more than 90% of RNA was absorbed by the pcMNPs within 10 min. In contrast, significant amount RNA molecules have been observed using a commercialized RNA extraction kit under similar conditions. More importantly, no aggregation has been observed when incubating pxMNPs in a high-salt solution (50 mM NaCl), further confirmed their excellent dispersity (Figure2B).
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