Author: Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong
Title: Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection Document date: 2019_12_23
ID: ahs2wdus_17
Snippet: An un-permeabilised control without secondary antibody was treated with cell 252 staining buffer and stained with intracellular antibodies. Cells were stained 253 with 125 nM Ir-Intercalator (Fluidigm) according to Fluidigm protocols and 254 fixed with 1.6% formaldehyde. Cells were counted on a BD Accuri C6. Before 255 acquisition on CyTOF Helios cytometer (Fluidigm), cells were re-suspended at 256 2×10 6 cells/mL in 0.1×EQ Four Element Calibra.....
Document: An un-permeabilised control without secondary antibody was treated with cell 252 staining buffer and stained with intracellular antibodies. Cells were stained 253 with 125 nM Ir-Intercalator (Fluidigm) according to Fluidigm protocols and 254 fixed with 1.6% formaldehyde. Cells were counted on a BD Accuri C6. Before 255 acquisition on CyTOF Helios cytometer (Fluidigm), cells were re-suspended at 256 2×10 6 cells/mL in 0.1×EQ Four Element Calibration Beads (Fluidigm). Data 257 files were processed and normalised using the CyTOF software v6.7 258 (Fluidigm). 259 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2019.12.20.885590 doi: bioRxiv preprint 260
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