Selected article for: "codon stop and stop codon"
Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo
  • Document date: 2020_4_5
    • ID: aju2nr9x_6
    Snippet: We next compared 3' UTR of the S27a with three alternative UTRs. Two 3' UTRs were obtained from the mRNAs of transferrin (TF) and α-1-antitrypsin (AAT), two abundant human plasma proteins 22 . Another 3' UTR from Hepatitis C viral RNA (HCV) was reported to retain and recycle the 40S subunit of ribosome after translation termination at stop codon, accelerating re-initiation of translation 23 . We then constructed these 3' UTRs with the same 5' UT.....
    Document: We next compared 3' UTR of the S27a with three alternative UTRs. Two 3' UTRs were obtained from the mRNAs of transferrin (TF) and α-1-antitrypsin (AAT), two abundant human plasma proteins 22 . Another 3' UTR from Hepatitis C viral RNA (HCV) was reported to retain and recycle the 40S subunit of ribosome after translation termination at stop codon, accelerating re-initiation of translation 23 . We then constructed these 3' UTRs with the same 5' UTR. As shown in Fig. 2b , these UTRs displayed similar effects on luciferase expression. Additionally, replacing S27a 3'UTR with another unstructured 3' UTR of the same length significantly decreased protein expression (Supplementary Table 3 and Supplementary Fig. 1 ). Since the length of S27a 3' UTR (34nt) was considerably shorter than other 3' UTRs (AAT 100nt, TF 386nt, or HCV 190nt), S27a 3' UTR was used in the following studies.

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