Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_60
Snippet: For completeness, an additional experiment was performed to generate the ribosome run-off curve from FLAG epitopes in the HA multi-frame tag. Because we can only image two epitopes at the same time (since RNA is imaged in a third color), we examined FLAG and Sun epitopes (Sun epitopes were required to distinguish frameshifting and nonframeshifting sites). The FLAG run-off from this experiment is on the right (green triangles). This curve is compa.....
Document: For completeness, an additional experiment was performed to generate the ribosome run-off curve from FLAG epitopes in the HA multi-frame tag. Because we can only image two epitopes at the same time (since RNA is imaged in a third color), we examined FLAG and Sun epitopes (Sun epitopes were required to distinguish frameshifting and nonframeshifting sites). The FLAG run-off from this experiment is on the right (green triangles). This curve is compared to the run-off curve of HA in HA only sites (orange squares) from Figure 4B . Both experiments represent non-frameshifting sites (no Sun signal detected). Error bars represent S.E.M.
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