Selected article for: "multi frame tag and scale bar"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_72
    Snippet: Single plane movies showing example U-2 OS cells expressing either +FSS multi-frame tag (left), -FSS control multi-frame tag (middle), or -FSS (+1 nt) tag with XXLb1 shifted into the 0 frame (right). All cells were bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, AlexX), and scFv-sfGFP (Ch3 blue, XXLb1). Images were acquired every 7 seconds for a total of 42 seconds. Scale bar, 10 µm. Figure 1D Max projection of a.....
    Document: Single plane movies showing example U-2 OS cells expressing either +FSS multi-frame tag (left), -FSS control multi-frame tag (middle), or -FSS (+1 nt) tag with XXLb1 shifted into the 0 frame (right). All cells were bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, AlexX), and scFv-sfGFP (Ch3 blue, XXLb1). Images were acquired every 7 seconds for a total of 42 seconds. Scale bar, 10 µm. Figure 1D Max projection of a 13 z-stack movie showing an example U-2 OS cell expressing the +FSS multi-frame tag and bead-loaded with probes: MCP-Halo JF646 (Ch1 red, RNA), FLAG Fab Cy3 (Ch2 green, 0 ORF), and scFv-sfGFP (Ch3 blue, -1 ORF). Images were acquired every 7 seconds for a total of 112 seconds prior to puromycin and 280 seconds following puromycin. Scale bar, 10 µm.

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