Lung epithelial cells have virus-specific and shared gene expression responses to infection by diverse respiratory viruses

James T. VanLeuven; Benjamin J. Ridenhour; Craig R. Miller; Tanya A. Miura

We used a murine lung epithelial cell line (LA4) to compare the gene expression response to these three 64 unrelated viruses. LA4 cells were derived from neoplastic lung epithelia from strain A (A/He) mice and have 65 some properties of alveolar type II cells [16] . Strain A (A/J) mice are highly susceptible to respiratory viral 66 infections, including MHV-1 and influenza A viruses [6, 10] . Other studies have demonstrated that LA4 cells 67 are susceptible to infection by PR8 and RV1B [15, 17] . In this study, we show that LA4 cells are also susceptible 68 to infection by MHV-1 (hereafter referred to as MHV). The gene expression response of LA4 cells to infection 69 by MHV, PR8, and RV1B (hereafter referred to as RV) differed in timing and magnitude of the changes. While Figure S1 ). Based on this, LA4 cells were 88 inoculated with 3 TCID50/cell RV, 1 PFU/cell PR8, or 3 PFU/cell MHV. Triplicate wells of LA4 cells in 6-well 89 plates were inoculated with each virus diluted in serum-free medium or were mock-inoculated with serum-free 90 medium for 1 h at 37ï‚°C. Viral inocula were removed and the cells were rinsed twice with serum-free medium.