SARS-CoV-2 RapidPlex: A Graphene-based Multiplexed Telemedicine Platform for Rapid and Low-Cost COVID-19 Diagnosis and Monitoring

Torrente-Rodríguez, Rebeca M.; Lukas, Heather; Tu, Jiaobing; Min, Jihong; Yang, Yiran; Xu, Changhao; Rossiter, Harry B.; Gao, Wei

The COVID-19 pandemic is an ongoing global challenge for public health systems. Ultrasensitive and early identification of infection is critical to prevent widespread COVID-19 infection by presymptomatic and asymptomatic individuals, especially in the community and in-home settings. We demonstrate a multiplexed, portable, wireless electrochemical platform for ultra-rapid detection of COVID-19: the SARS-CoV-2 RapidPlex. It detects viral antigen nucleocapsid protein, IgM and IgG antibodies, as well as the inflammatory biomarker C-reactive protein, based on our mass-producible laser-engraved graphene electrodes. We demonstrate ultrasensitive, highly selective, and rapid electrochemical detection in the physiologically relevant ranges. We successfully evaluated the applicability of our SARS-CoV-2 RapidPlex platform with COVID-19 positive and negative blood and saliva samples. Based on this pilot study, our multiplexed immunosensor platform may allow for high frequency at-home testing for COVID-19 telemedicine diagnosis and monitoring.