Author: Sandra De Meyer; Denisa Bojkova; Jindrich Cinati; Ellen Van Damme; Christophe Buyck; Marnix Van Loock; Brian Woodfall; Sandra Ciesek
Title: Lack of Antiviral Activity of Darunavir against SARS-CoV-2 Document date: 2020_4_8
ID: 679qfp2s_11
Snippet: Human colon carcinoma cell line (Caco-2) cells (obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany) were cultured in Minimal Essential Medium (MEM) supplemented with 10% fetal bovine serum (FBS) and containing penicillin (100 IU/mL) and streptomycin (100 μ g/mL) in a 5% CO 2 atmosphere at 37°C. All culture reagents were purchased from Sigma (Hamburg, Germany). SARS-CoV-2 was isolated from human sampl.....
Document: Human colon carcinoma cell line (Caco-2) cells (obtained from the Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany) were cultured in Minimal Essential Medium (MEM) supplemented with 10% fetal bovine serum (FBS) and containing penicillin (100 IU/mL) and streptomycin (100 μ g/mL) in a 5% CO 2 atmosphere at 37°C. All culture reagents were purchased from Sigma (Hamburg, Germany). SARS-CoV-2 was isolated from human samples and cultured in Caco-2 cells, as previously described (3). After one passage in Caco-2 cells, viral stocks were stored at -80°C prior to use.
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