Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_157
Snippet: Relating model dynamics to experimental fluorescence intensity. To relate ribosome occupancy to experimental translation spots, we proscribe an assumed fluorescence to each ribosome based upon its position. This intensity is proportional to the number of peptide probes upstream from the ribosome location and in the appropriate frame or frames. Ribosomes in the 0 frame include all upstream probes in the 0 frame. Ribosomes in the -1 frame include p.....
Document: Relating model dynamics to experimental fluorescence intensity. To relate ribosome occupancy to experimental translation spots, we proscribe an assumed fluorescence to each ribosome based upon its position. This intensity is proportional to the number of peptide probes upstream from the ribosome location and in the appropriate frame or frames. Ribosomes in the 0 frame include all upstream probes in the 0 frame. Ribosomes in the -1 frame include probes in the 0 frame between the start codon and the FSS (if any) and probes in the -1 frame between the FSS and the current ribosome position. The total intensity vector for the â„Ž color is given by
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