Mammalian orthoreovirus infection is enhanced in cells pre-treated with sodium arsenite

Michael M. Lutz; Megan P. Worth; Meleana M. Hinchman; John S. L. Parker; Emily D. Ledgerwood

permissive for viral replication [12, 13] . However, not all viruses benefit from inhibition 105 of kinase activity and initiation of the ISR. A study examining the role of the PKR-eIF2 106 pathway during mammalian orthoreovirus (reovirus) infection found some strains had 107 reduced titers in PKR knockout murine embryonic fibroblasts (MEF) [14] . Follow-up 108 studies observed both increased ISR gene expression and reduced levels of the eIF2α 109 kinase inhibitor P58 IPK in cells infected with reovirus strains known to robustly interfere 110 with host translation, and these strains replicated less efficiently in MEFs expressing a 111 non-phosphorylatable form of eIF2α [15] . 112 infection, the stress granules have disappeared and viral factories (VFs), the sites of 116 prototype reovirus serotype 3 strain Abney (T3A) was a kind gift from Dr. Barbary 162 Sherry (North Carolina State University) and was plaque-purified and passaged twice on 163 L-cell monolayers to generate working stocks. 164 165 Infections. CV-1, L929, HeLa or HPDE cells were seeded in 24-well culture plates 166 containing 12 mm glass coverslips or 12-well culture plates the day before to give rise to 167 50 to 80% confluence prior to infection. Cells were infected with virus at the indicated 168 MOI for 1 h at room temperature (RT) in phosphate-buffered saline (PBS; pH 7.4), 169