One-for-All Nanoplatform for Synergistic Mild Cascade Potentiated Ultrasound Therapy Induced with Targeting-Imaging-Guided Photothermal Therapy.

Cheng, Kai; Zhang, Ruo-Yun; Yang, Xiao-Quan; Zhang, Xiao-Shuai; Zhang, Fang; An, Jie; Wang, Zhuo-Ya; Dong, Ying; Liu, Bo; Zhao, Yuan-Di; Liu, Tiancai

Ameliorated therapy based on the tumor microenvironment is becoming increasingly popular, yet only few methods have achieved wide recognition. Herein, targeting multifunctional hydrophilic nanomicelles, AgBiS2@DSPE-PEG2000-FA (ABS-FA), were obtained and employed for tumor treatment. In a cascade amplification mode, ABS-FA exhibited favorable properties of actively enhancing Computed Tomography/Infrared (CT/IR) imaging and gently relieving ambient oxygen concentration by cooperative photothermal and sonodynamic therapy. Compared with traditional Bi2S3 nanoparticles, the CT imaging capability of the probe was augmented (43.21%), and the photothermal conversion efficiency was increased (33.1%). Furthermore, remarkable ultrasonic dynamics features of ABS-FA were observed, with increased generation of reactive oxygen species (ROS) (24.3%) being obtained compared to Ce6, a commonly used sonosensitizer. Furthermore, ABS-FA exhibited obvious inhibitory effects on HeLa cell migration at 6 μg/mL, which to some extent demonstrated its suppressive effect on tumor growth. A lower dose, laser and ultrasonic power, and a shorter processing time endowed ABS-FA with excellent photothermal and sonodynamic effects. By mild cascade mode, the hypoxic condition of the tumor site was largely improved, and a suitable oxygen-rich environment was provided, thereby endowing ABS-FA with a superior synergistic enhanced treatment effect compared with the single mode approach, which ultimately realized the purpose of "one injection, multiple treatment". Moreover, our data showed that ABS-FA was given with a biological safety profile while harnessed in vivo. Taken together, as a synergistically-enhanced medical diagnosis and treatment method, the One-for-All nanoplatform will pave a new avenue for further clinical applications.