Author: Michael M. Lutz; Megan P. Worth; Meleana M. Hinchman; John S. L. Parker; Emily D. Ledgerwood
Title: Mammalian orthoreovirus infection is enhanced in cells pre-treated with sodium arsenite Document date: 2019_2_20
ID: cpfwzudq_4
Snippet: supplemented with 2 mM MgCl 2 , with rocking every 10 min. Following absorption, virus 170 was removed and cells were incubated in growth medium at 37°C and harvested at the conflicting as to the timing of stress granule presence in infected cells [15, 16] . To 230 evaluate this further, we first confirmed that stress granules could form in CV-1 cells by 231 treating uninfected cells with 0.5 mM sodium arsenite for 30 min. We then fixed and co-2.....
Document: supplemented with 2 mM MgCl 2 , with rocking every 10 min. Following absorption, virus 170 was removed and cells were incubated in growth medium at 37°C and harvested at the conflicting as to the timing of stress granule presence in infected cells [15, 16] . To 230 evaluate this further, we first confirmed that stress granules could form in CV-1 cells by 231 treating uninfected cells with 0.5 mM sodium arsenite for 30 min. We then fixed and co-232 immunostained for TIAR and G3BP, two RNA binding proteins required for stress 233 granule formation. We saw stress granules in ~91% of treated CV-1 cells, confirming that 234 these cells can form stress granules ( Figure 1A , upper panels). We next infected CV-1 235 cells with reovirus at a multiplicity of infection (MOI) of 10 and fixed and 236 immunostained cells at the indicated times post-infection (p.i.) for the presence of stress 237 granules (TIAR), and viral factories (µNS) ( Figure 1A , lower panels). Stress granules 238
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