Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_31
Snippet: To quantify the kinetics of frameshifting, we developed two candidate models and attempted to fit each to our three main observations: (i) the percentages of frameshifting versus non-frameshifting translation sites (Fig. 4C ), (ii) ribosomal occupancies (Fig 4D) , and (iii) run-off kinetics (Figs. 4E,F). Both models include initiation of ribosomes, codondependent elongation of proteins along the RNA template, and ribosomal exclusion to block ribo.....
Document: To quantify the kinetics of frameshifting, we developed two candidate models and attempted to fit each to our three main observations: (i) the percentages of frameshifting versus non-frameshifting translation sites (Fig. 4C ), (ii) ribosomal occupancies (Fig 4D) , and (iii) run-off kinetics (Figs. 4E,F). Both models include initiation of ribosomes, codondependent elongation of proteins along the RNA template, and ribosomal exclusion to block ribosomes from passing one another or occupying the same place on the RNA.
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