Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting Document date: 2018_11_24
ID: 4fm1skgh_35
Snippet: To estimate the timescale of and , we tracked translation sites for longer periods of time. To achieve this tracking, we doubled the number of epitopes in the multiframe reporter (creating the +FSS 2x multi-frame tag) and changed our imaging strategy to sample the RNA signal at all time points and the 0 and -1 translation signals once every fifth time point. This arrangement substantially reduced photobleaching and allowed us to continuously trac.....
Document: To estimate the timescale of and , we tracked translation sites for longer periods of time. To achieve this tracking, we doubled the number of epitopes in the multiframe reporter (creating the +FSS 2x multi-frame tag) and changed our imaging strategy to sample the RNA signal at all time points and the 0 and -1 translation signals once every fifth time point. This arrangement substantially reduced photobleaching and allowed us to continuously track and monitor the translational status of single translation sites in 3D
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