Selected article for: "multi frame tag and reverse multi frame tag"
Author: Kenneth Lyon; Luis U. Aguilera; Tatsuya Morisaki; Brian Munsky; Timothy J. Stasevich
Title: Live-cell single RNA imaging reveals bursts of translational frameshifting
  • Document date: 2018_11_24
    • ID: 4fm1skgh_95
    Snippet: The HIV-1 frameshift sequence (FSS) followed by either the multi-frame (MF) tag or the reverse multi-frame (revMF) tag were synthesized by GeneArt® gene synthesis service (Thermo Fisher Scientific). The gene fragments were flanked by NotI and NheI, and fused to the upstream of the beta-actin zipcode and 24x MS2 stem loops in the 3' UTR of plasmid pUB_smFLAG_ActB_MS2 (Plasmid #81083, addgene) to obtain the FSS-MF and FSS-revMF, respectively. To d.....
    Document: The HIV-1 frameshift sequence (FSS) followed by either the multi-frame (MF) tag or the reverse multi-frame (revMF) tag were synthesized by GeneArt® gene synthesis service (Thermo Fisher Scientific). The gene fragments were flanked by NotI and NheI, and fused to the upstream of the beta-actin zipcode and 24x MS2 stem loops in the 3' UTR of plasmid pUB_smFLAG_ActB_MS2 (Plasmid #81083, addgene) to obtain the FSS-MF and FSS-revMF, respectively. To double the MF tag, the MF tag region was digested out from FSS-MF with XbaI and AgeI, and then ligated into FSS-MF flanked with NheI and

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