Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_1
Snippet: transcription polymerase chain reaction (RT-PCR) is identified as a standard and routinely used technique for the analysis and quantification of various pathogenic RNA in laboratories and clinical diagnosis. 2 For example, it has been successfully applied for the detection of SARS-CoV, Middle East respiratory syndrome coronavirus (MERS-CoV), and various other viral pathogens including Zika virus (ZIKV), Influenza A virus, Dengue virus (DENV). [3].....
Document: transcription polymerase chain reaction (RT-PCR) is identified as a standard and routinely used technique for the analysis and quantification of various pathogenic RNA in laboratories and clinical diagnosis. 2 For example, it has been successfully applied for the detection of SARS-CoV, Middle East respiratory syndrome coronavirus (MERS-CoV), and various other viral pathogens including Zika virus (ZIKV), Influenza A virus, Dengue virus (DENV). [3] [4] [5] [6] [7] [8] After the outbreak of COVID-19, several methods and kits based on RT-PCR for the detection of SARS-CoV-2 genomic RNA have been reported. [9] [10] While RT-PCR-based methods have been widely used in COVID-19 diagnosis, their application in accurate diagnosis of viral infection and epidemic control is severely hampered by their laborious and time-consuming sample processing steps.
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