Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_23
Snippet: To investigate whether the extracted pcMNPs-RNA complexes can be directly used for RT-PCR, an identical extraction was carried out, in which the whole complex was transferred to the PCR tube without the elution step. Specifically, 30 μL of reaction solution composed of 15 μL of TE buffer, 7.5 μL of 4 × reaction buffer, 1.5 μL enzyme mixture, and optimal concentrations of primers (1 μM each) and probe (1 μM) was mixed with pcMNP-RNA complex.....
Document: To investigate whether the extracted pcMNPs-RNA complexes can be directly used for RT-PCR, an identical extraction was carried out, in which the whole complex was transferred to the PCR tube without the elution step. Specifically, 30 μL of reaction solution composed of 15 μL of TE buffer, 7.5 μL of 4 × reaction buffer, 1.5 μL enzyme mixture, and optimal concentrations of primers (1 μM each) and probe (1 μM) was mixed with pcMNP-RNA complexes by brief vortexing, and directly transferred to the PCR tube for RT-PCR using the same procedure as abovementioned.
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