Selected article for: "fish hybridization and situ fish hybridization"

Author: Chad N. Brocker; Donghwan Kim; Tisha Melia; Kritika Karri; Thomas J. Velenosi; Shogo Takahashi; Jessica A. Bonzo; David J. Waxman; Frank J. Gonzalez
Title: Long non-coding RNA Gm15441 attenuates hepatic inflammasome activation in response to metabolic stress
  • Document date: 2019_6_20
  • ID: dt0b7jnu_71
    Snippet: For fluorescence in situ hybridization (FISH) staining of Gm15441, Gm15441 +/+ and Gm15441 LSL mice were fed either a control diet or diet containing WY-14643 for 36 hours. Livers were harvested, fixed in 4% of paraformaldehyde overnight then sent to the Molecular Pathology Laboratory (PHL) at the National Cancer Institute for processing. RNA FISH experiments were performed by the using custom RNAscope probes and reagents developed by Advanced Ce.....
    Document: For fluorescence in situ hybridization (FISH) staining of Gm15441, Gm15441 +/+ and Gm15441 LSL mice were fed either a control diet or diet containing WY-14643 for 36 hours. Livers were harvested, fixed in 4% of paraformaldehyde overnight then sent to the Molecular Pathology Laboratory (PHL) at the National Cancer Institute for processing. RNA FISH experiments were performed by the using custom RNAscope probes and reagents developed by Advanced Cell Diagnostics (Newark, CA). Proprietary FISH probes targeted a region of Gm15441 that does not overlap with Txnip to prevent signal interference (Table S5) . Slide imaging was performed using Aperio ImageScope software (Leica Biosystems, Buffalo Grove, IL, USA).

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