A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2

Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu

author/funder. All rights reserved. No reuse allowed without permission. In these experiments, the serum sample without pseudoviruses was used as a negative control, while the PCR reaction mixture directly spiked with 10 5 copies of pseudoviruses was regarded as a positive control. As shown in Figure3A The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint eluted products was added for the Conventional RT-PCR. This phenomenon suggests that one advantages of the pcMNPs-based Direct RT-PCR protocol is to reduce possible false negative results, since all extracted viral RNA has been directed to the subsequent amplification without potential lost in the elution and transfer steps. Additionally, there is no detectable differences between amplification efficiencies of the positive control and Direct RT-PCR (Figure3A red and purple lines) . This result suggests that our pcMNPs-based viral RNA extraction protocol not only exhibits nearly 100% RNA extraction efficiency in serum samples, but also provides high-purity products without PCR inhibitors.