Author: Ioanna Smyrlaki; Martin Ekman; Martin Vondracek; Natali Papanicoloau; Antonio Lentini; Johan Aarum; Shaman Muradrasoli; Jan Albert; Björn Högberg; Björn Reinius
Title: Massive and rapid COVID-19 testing is feasible by extraction-free SARS-CoV-2 RT-qPCR Document date: 2020_4_17
ID: jeufhpkq_11
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.04.17.20067348 doi: medRxiv preprint hid-RT-qPCR (by both N1 and RdRP) but were negative in routine diagnostics on extracted RNA (Fig. 3a , sample 27, 37 and 62). Of note, two of these patients were re-tested by collection of a new swab sample in clinic 2 and 4 days after the original routine diagnostics and were then confirmed to be COVID-19 po.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is . https://doi.org/10.1101/2020.04.17.20067348 doi: medRxiv preprint hid-RT-qPCR (by both N1 and RdRP) but were negative in routine diagnostics on extracted RNA (Fig. 3a , sample 27, 37 and 62). Of note, two of these patients were re-tested by collection of a new swab sample in clinic 2 and 4 days after the original routine diagnostics and were then confirmed to be COVID-19 positive (CT 26-27, E and RdRP). Corrected N1 hid-RT-qPCR accuracy: 94% CI95%: 87-98%, sensitivity: 91% and specificity: 97%, when taking re-testing into account ( Table 2) .
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