Author: Chunxi Zeng; Xucheng Hou; Jingyue Yan; Chengxiang Zhang; Wenqing Li; Weiyu Zhao; Shi Du; Yizhou Dong
Title: Leveraging mRNAs sequences to express SARS-CoV-2 antigens in vivo Document date: 2020_4_5
ID: aju2nr9x_13
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.01.019877 doi: bioRxiv preprint expression than NCA-7, while NCA-8d was less active than NCA-8 (Fig. 3c) . Based on these results, NCA-7d was selected as the lead 5' UTR. UTRs in Hep 3B and 293T cells. c, Relative luciferase activity after removal of miRNA target sites in 5' UTR. NCA-7d and NCA-8d: removal of miRNA binding sites from NCA-.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.04.01.019877 doi: bioRxiv preprint expression than NCA-7, while NCA-8d was less active than NCA-8 (Fig. 3c) . Based on these results, NCA-7d was selected as the lead 5' UTR. UTRs in Hep 3B and 293T cells. c, Relative luciferase activity after removal of miRNA target sites in 5' UTR. NCA-7d and NCA-8d: removal of miRNA binding sites from NCA-7 and NCA-8, respectively. Relative luciferase activity was normalized to that of CYBA. All data are presented as the mean ± s.d. (n=4).
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