Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_38
Snippet: Overall, the updated MB-based extraction method had highly extraction efficiency and compatibility of PCR amplification in any of the patterns, which dramatically simplified laborious sample processing work and was ideally suitable for RT-PCR assay of SARS-CoV-2 with a sensitivity of 10 copies at least. Efficient and robust nucleic acids extraction from complex clinical samples is the first and the most important step for subsequent molecular dia.....
Document: Overall, the updated MB-based extraction method had highly extraction efficiency and compatibility of PCR amplification in any of the patterns, which dramatically simplified laborious sample processing work and was ideally suitable for RT-PCR assay of SARS-CoV-2 with a sensitivity of 10 copies at least. Efficient and robust nucleic acids extraction from complex clinical samples is the first and the most important step for subsequent molecular diagnosis, but currently it is still highly labour intensive and time-consuming. For example, although the genome author/funder. All rights reserved. No reuse allowed without permission.
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