Author: Zhen Zhao; Haodong Cui; Wenxing Song; Xiaoling Ru; Wenhua Zhou; Xuefeng Yu
Title: A simple magnetic nanoparticles-based viral RNA extraction method for efficient detection of SARS-CoV-2 Document date: 2020_2_27
ID: nbu6bsb3_40
Snippet: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint sequences of SARS-CoV2 have been fully revealed and various RT-PCR-based detection kits have been developed, timely diagnosis of COVID-19 is still highly challenging partially due to the lack of satisfactory viral RNA extraction strategy. In this study, a carboxyl polymer-coated MNPs, namely pcMNPs, was dev.....
Document: The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2020.02.22.961268 doi: bioRxiv preprint sequences of SARS-CoV2 have been fully revealed and various RT-PCR-based detection kits have been developed, timely diagnosis of COVID-19 is still highly challenging partially due to the lack of satisfactory viral RNA extraction strategy. In this study, a carboxyl polymer-coated MNPs, namely pcMNPs, was developed and a simple but efficient viral RNA extraction system was established for sensitive detection of SARS-CoV-2 RNA via RT-PCR. As compared with traditional column-based nucleic acids extraction methods, our pcMNPs-based method has several advantages ( Table 2) .
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