Author: Haran Shani-Narkiss; Omri David Gilday; Nadav Yayon; Itamar Daniel Landau
Title: Efficient and Practical Sample Pooling High-Throughput PCR Diagnosis of COVID-19 Document date: 2020_4_7
ID: 6ji8dkkz_6
Snippet: In practice, if the presence of amplified viral cDNA is detected in a significant amount before a certain cycle number (e.g. 30), and given that the human cDNA (extraction control) was also detected in the sample, the patient is declared positive. If amplified viral cDNA is not detected or only detected in very late cycles (e.g. 40) then the patient is declared negative......
Document: In practice, if the presence of amplified viral cDNA is detected in a significant amount before a certain cycle number (e.g. 30), and given that the human cDNA (extraction control) was also detected in the sample, the patient is declared positive. If amplified viral cDNA is not detected or only detected in very late cycles (e.g. 40) then the patient is declared negative.
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