Evaluation of an immunochemotherapeutic protocol constituted of N-methyl meglumine antimoniate (Glucantime(®)) and the recombinant Leish-110f(®) + MPL-SE(®) vaccine to treat canine visceral leishmaniasis

Miret, Jorge; Nascimento, Evaldo; Sampaio, Weverton; França, João Carlos; Fujiwara, Ricardo Toshio; Vale, André; Dias, Edelberto Santos; Vieira, Edvá; Costa, Roberto Teodoro da; Mayrink, Wilson; Neto, Antonio Campos; Reed, Steven

The evaluation of the efficacy of an immunochemotherapy protocol to treat symptomatic dogs naturally infected with Leishmania chagasi was studied. This clinical trial had the purpose to test the combination of N-methyl meglumine antimoniate (Glucantime(®)) and the second generation recombinant vaccine Leish-110f(®) plus the adjuvant MPL-SE(®) to treat the canine leishmaniasis (CanL). Thirty symptomatic naturally infected mongrel dogs were divided into five groups. Animals received standard treatment with Glucantime(®) or treatment with Glucantime(®)/Leish-110f(®) + MPL-SE(®) as immunochemotherapy protocol. Additional groups received Leish-110f(®) + MPL-SE(®) only, MPL-SE(®) only, or placebo. Evaluation of haematological, biochemical (renal and hepatic function) and plasmatic proteins, immunological (humoral and cellular immune response) and the parasitological test revealed improvement of the clinical parameters and parasitological cure in dogs in both chemotherapy alone and immunochemotherapy cohorts. However, the immunotherapy and immunochemotherapy cohorts had reduced number of deaths, higher survival probability, and specific cellular reactivity to leishmanial antigens, in comparison with chemotherapy cohort only and control groups (adjuvant alone and placebo). These results support the notion of using well-characterized recombinant vaccine as an adjunct to improve the current chemotherapy of CanL.