Author: Zoltick, Philip W.; Leibowitz, Julian L.; Oleszak, Emilia L.; Weiss, Susan R.
Title: Mouse hepatitis virus ORF 2a is expressed in the cytosol of infected mouse fibroblasts Cord-id: hgjwn1l6 Document date: 2004_2_6
ID: hgjwn1l6
Snippet: A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17CI-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.
Document: A 266-bp fragment of cDNA from within gene B, ORF 2a, of MHV-A59 was used to construct a vector encoding a bacterial/viral fusion protein. Antiserum raised against this fusion protein specifically immunoprecipitates a 30K protein from infected 17CI-1 mouse fibroblasts. The protein is localized primarily in the cytosol and not in the membranes. This is consistent with its predicted sequence and potential role as an RNA binding protein.
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