Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection

Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong

Singlets were identified from 191Ir v event length plots; Live cells were 191Ir + 264 195Pt -. For lung tissue samples, epithelial cells were identified as CD45 -265 EpCAM + . For cord blood a further gating step was performed to separate the 266 CD34 + cells from the CD34cells. CD34 + cells were further gated to identify 267 the various progenitor populations: HSC CD38 -CD90 + ; MPP CD38 -CD90 -; 268 The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10.1101/2019.12.20.885590 doi: bioRxiv preprint three Eppendorf tubes: one was immediately stained for IFITM3 expression 294 and run on a flow cytometer (Attune NxT); one was infected with S-FLU 295 (PR8:H1N1) at a ratio of 1:4; and the other was left uninfected. Cells were 296 plated into 24 well dishes for 24 hours. The level of IFITM3 expression and 297 GFP + percentage of cells was determined for each condition. Data was 298 analysed by linear correlation on GraphPad Prism. 299 300 Results 301