Low basal expression and slow induction of IFITM3 puts immune cells at risk of influenza A infection

Dannielle Wellington; Zixi Yin; Liwei Zhang; Jessica Forbester; Kerry Kite; Henry Laurenson-Schafer; Shokouh Makvandi-Nejad; Boquan Jin; Emma Bowes; Krishnageetha Manoharan; David Maldonado-Perez; Clare Verill; Ian Humphreys; Tao Dong

suggesting that the IFITM3 expression pattern is more complicated than 356 previously thought. The expression pattern on most immune cell subsets was 357 comparable between adult and cord blood samples, with the highest 358 expression by CD16 + monocytes (Figure 1e) . In general, IFITM3 expression 359 was higher in cord blood samples than comparable populations in adult blood 360 samples suggesting that naïve cells may have higher expression of IFITM3. 361 . CC-BY-NC-ND 4.0 International license author/funder. It is made available under a The copyright holder for this preprint (which was not peer-reviewed) is the . https://doi.org/10. 1101 /2019 As opposed to IFITM3 expression gradually reducing with increased 362 differentiation, we saw a distinct pattern of IFITM3 expression across the HSC 363 differentiation pathway (Figure 2) . This suggests that while differentiation 364 state is a determinate of basal IFITM3 expression it is not a linear correlation. 365 366