Selected article for: "catalytic activity and crystal structure"
Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection
  • Document date: 2020_4_14
    • ID: n5kpvsvg_1
    Snippet: Based on the crystal structure of LbCas12a/crRNA/dsDNA (PDB ID: 5XUS) 12 , we reasoned that crRNA extensions can influence the trans-cleavage activity by either activating or inhibiting the catalytic efficiency of Cas12a, allowing us to better understand crRNA design with tunable transcleavage activity. We speculate that chemical modifications of the crRNA can potentially change its nature of binding and subsequently alter this collateral cleavag.....
    Document: Based on the crystal structure of LbCas12a/crRNA/dsDNA (PDB ID: 5XUS) 12 , we reasoned that crRNA extensions can influence the trans-cleavage activity by either activating or inhibiting the catalytic efficiency of Cas12a, allowing us to better understand crRNA design with tunable transcleavage activity. We speculate that chemical modifications of the crRNA can potentially change its nature of binding and subsequently alter this collateral cleavage due to conformational changes of the Cas12a dynamic endonuclease domain.

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