Author: Long T. Nguyen; Brianna M Smith; Piyush K. Jain
Title: Enhancement of trans-cleavage activity of Cas12a with engineered crRNA enables amplified nucleic acid detection Document date: 2020_4_14
ID: n5kpvsvg_28
Snippet: The resulting fraction was equilibrated with buffer C (100 mM NaCl, 20 mM HEPES, 0.5 mM TCEP, PH = 8) at a 1:1 ratio and run through Hitrap Heparin HP 1 ml column (GE Biosciences). The column was washed with buffer C and gradually eluted at a gradient rate with buffer D (100 mM NaCl, 20 mM HEPES, 0.5 mM TCEP, PH = 8). The eluted fraction was concentrated down to 500 ïL and passed through the Hiload Superdex 200 pg column (GE Biosciences). The p.....
Document: The resulting fraction was equilibrated with buffer C (100 mM NaCl, 20 mM HEPES, 0.5 mM TCEP, PH = 8) at a 1:1 ratio and run through Hitrap Heparin HP 1 ml column (GE Biosciences). The column was washed with buffer C and gradually eluted at a gradient rate with buffer D (100 mM NaCl, 20 mM HEPES, 0.5 mM TCEP, PH = 8). The eluted fraction was concentrated down to 500 ïL and passed through the Hiload Superdex 200 pg column (GE Biosciences). The purified LbCas12a was then buffer exchanged with storage buffer (500 mM NaCl, 20 mM Na2CO3, 0.1mM TCEP, 50% glycerol, PH = 6) and flash frozen at -80 o C until use.
Search related documents:
Co phrase search for related documents- ml column and mm hepes: 1
- mm hepes and pg column: 1
- mm hepes and storage buffer: 1, 2, 3
Co phrase search for related documents, hyperlinks ordered by date