Selected article for: "bovine serum and minimum MEM essential medium"

Author: Jacob Peter Matson; Amy M. House; Gavin D. Grant; Huaitong Wu; Joanna Perez; Jeanette Gowen Cook
Title: Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence
  • Document date: 2019_2_22
  • ID: dsbucda9_33
    Snippet: RPE1-hTERT cells, 293T and NHF1-hTERT cells (ATCC) were grown in Dulbecco's modified Eagle's medium (DMEM) (Sigma Aldrich) with 10% Fetal Bovine Serum (FBS) (Seradigm) and 2 mM L-glutamine (Gibco) at 37°C with 5% CO2. Wi38 cells (ATCC) were grown in Minimum Essential Medium (MEM) with 10% FBS (Seradigm), 2 mM L-glutamine (Gibco) and Minimum Essential Medium non-essential amino acids (NEAA) (Gibco) at 37°C with 5% CO2. Cells were passaged every .....
    Document: RPE1-hTERT cells, 293T and NHF1-hTERT cells (ATCC) were grown in Dulbecco's modified Eagle's medium (DMEM) (Sigma Aldrich) with 10% Fetal Bovine Serum (FBS) (Seradigm) and 2 mM L-glutamine (Gibco) at 37°C with 5% CO2. Wi38 cells (ATCC) were grown in Minimum Essential Medium (MEM) with 10% FBS (Seradigm), 2 mM L-glutamine (Gibco) and Minimum Essential Medium non-essential amino acids (NEAA) (Gibco) at 37°C with 5% CO2. Cells were passaged every three days with trypsin (Sigma Aldrich) and not allowed to reach confluency.

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