Author: Jacob Peter Matson; Amy M. House; Gavin D. Grant; Huaitong Wu; Joanna Perez; Jeanette Gowen Cook
Title: Intrinsic checkpoint deficiency during cell cycle re-entry from quiescence Document date: 2019_2_22
ID: dsbucda9_35
Snippet: To synchronize RPE1 in 3 repeated G0s, cells were synchronized in G0 by contact inhibition (above), re-stimulated into the cell cycle by passaging 1:6 in DMEM with 10% FBS and 2 mM L-glutamine, and grown to 100% confluency within 48-72 hours to start the second G0 by contact inhibition for 48 hours. Cells were re-stimulated as before and grown to 100% confluency to start the 3 rd G0 for 48 hours. To release cells for the experiment, G0 cells were.....
Document: To synchronize RPE1 in 3 repeated G0s, cells were synchronized in G0 by contact inhibition (above), re-stimulated into the cell cycle by passaging 1:6 in DMEM with 10% FBS and 2 mM L-glutamine, and grown to 100% confluency within 48-72 hours to start the second G0 by contact inhibition for 48 hours. Cells were re-stimulated as before and grown to 100% confluency to start the 3 rd G0 for 48 hours. To release cells for the experiment, G0 cells were re-stimulated by passaging 1:10 in DMEM with 10% FBS and 2 mM L-glutamine, harvesting the first cell cycle at 24 hours after restimulation.
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